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Immediately extracted from the trace elements and lipids by the use of solid phase extraction CHROMABOND column NH2 phase Cat. No. 730031. The sample was then injected directly into an AQUA column 150 54.6 mm 5 C18, purchased from Phenomenex, USA under the following conditions: mobile phase 97/3 20 Mm potassium phosphate, pH 3.0/ methanol, flow rate 1.5 ml/min, UV 270 nm. norepinephrine, dopamine