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Ly Research Laboratories, Indianapolis, IN, USA) were made in dimethyl sulfoxide and diluted in medium, i.e., DMEM with 2.5 FBS, before use. Stock solutions of 10 mg/ml 2-AP; 4 mg/ml levamisole; 10 mg/ml L-NAME and 1 mg/ml L-NMMA were directly made in medium; hemin: a stock solution of 3.25 mg/ml was prepared by addingPage 8 of(page number not for citation purposes)Virology Journal 2008, 5:http:/
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Ed. RAW, DRAW and CDRAW cells were grown in Dulbecco's modified Eagle medium (DMEM) with 2.5 fetal bovine serum (FBS).L929 cells, originally derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse and purchased from ATCC, were used for plaque assay. Cells were grown as monolayers in minimal essential medium supplemented with Earle's salts, nonessential amino
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E been identified by MALDI-ToF/ToF are circled and numbered and all proteins are named in Table 1. Note that in cases where multiple isoforms are circled, the identity of all isoforms has been confirmed by MALDI-ToF/ToF.below those seen in the corresponding infected WT mice, resulting in a regulatory pattern that is almost the inverse of that described above for most of the proteins. These protein
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Cence detection.Phase-contrast microscopy Phase-contrast microscopy was performed with a Zeiss Axiovert100 microscope and photomicrographs were taken with an AxioCam MRc5 digital camera.9.10.11.12.Competing interestsThe authors declare that they have no competing interests.13.Authors' contributionsSS participated in the experimental design, implementation and interpretation of results and performe
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Stimulated M2 polarization. Moreover, human explant mammary gland cultures treated with rSFRP1 show a marked reduction in the human M2 marker, CD163. CD163 is a monocyte/macrophage-restricted scavenge receptor [47] and the mechanism by which rSFRP1 reduces CD163 expression may be due in part to repression of Wnt signaling because Bergenfelz et al. show that breast cancer CD163+ TAMs correlate with